Single-cell CSI: Development and application of single-cell DNA profiling in forensic science

Every nucleated cell has the potential to identify us. We shed cells wherever we go and different types of contact result in the transfer and mixture of different cell types.

Conventional forensic DNA recovery loses information about the molecule – or cell – the DNA originated from. This information could be critical in mixed samples and establishing where a DNA molecule came from, when it was transferred, and by whom. This project will combine advances in single-cell isolation, phenotyping, and sequencing with established and novel approaches for human identification from individual cells. Using advanced cell sorting, laser capture microdissection and microfluidic approaches, the student will develop and apply methods for human identification from individual cells.

The student will apply short-tandem repeat (STR)-based analysis methods and sequencing approaches to single cells. The project will focus on method development with translational potential for the justice system but collaboration with the Nieduszynski group will also explore STR heterogeneity in normal human biology

We offer a highly collaborative research environment, exceptionally well equipped for single-cell genomics. The project offers experience in highly sought after skills in cell biology, genomics and technology development. The student will be mentored to pursue independent lines of research and gain visibility in the wider research community.

The project has collaborative links with researchers and practitioners in UK forensic science who will provide guidance on technical developments and their applicability in real-world forensic settings.

References

Single cell genomics applications in forensic science: Current state and future directions, iScience. 2023 Nov 17; 26(11): 107961.

Revisiting single cell analysis in forensic science, Scientific Reports volume 11, Article number: 7054 (2021)