Investigating genetic markers and mechanisms of CO dehydrogenase regulation in response to atmospheric mercury. (HERNANDEZ GARCIA_U26DTP)

Scientific Background
Volcanic emissions are a natural source of mercury (Hg) to the atmosphere and Mt. Etna is a major natural source of Hg to the Mediterranean region. Previous research has shown that Hg deposited on the low pH and organic-poor soils of Etna’s south-eastern flank may not be retained but instead released to groundwater or re-emitted to the atmosphere, indicating that the deposition of volcanic Hg has clear environmental impacts. The potential for Hg to disrupt local ecosystems is particularly concerning, as it may alter microbial activity, including their capability to oxidise trace gases such as carbon monoxide (CO), also abundant at Etna volcano. This plays a crucial role in atmospheric chemistry and climate regulation. In this project, you will investigate if Hg affects the microbial communities and specifically their capability to oxidise trace gases, such as CO.

The project
You will be answering these key questions: 1) How do bacterial communities in recently formed soils from Mt Etna utilise CO, and what specific metabolic pathways are involved? and 2) Does exposure to mercury influence the expression and activity of the CO-dehydrogenase enzyme in bacteria isolated from Mt. Etna soils?
To answer these questions, you will join sampling campaigns at Mt. Etna followed by soil incubations and trace-gas measurements. You will be trained in cutting-edge tools for isolation of microbes, including whole genome sequencing, proteomics, and metagenomics.

Training
Specific training will include: the cultivation of soil bacteria; quantitative PCR; high-throughput sequencing and proteomics. You will present results at weekly lab meetings, departmental seminars, and at national and international conferences.

Person specification
We are looking for a pro-active, highly motivated student willing to join sampling campaigns at Etna Volcano. The candidate should have a strong background in microbiology, biochemistry, soil or environmental sciences (BSc/Masters in Soil Microbiology, Environmental Microbiology, or similar).