Engineering biology to decode gene regulatory networks in lateral root initiation (GUIZIOU_E27DTP)

Are you a motivated undergraduate with a passion for engineering biology and plant science?
We’re offering an exciting PhD opportunity with the Guiziou Group at the Earlham Institute using engineering biology and single-cell genomic technology to drive forward our understanding of lateral root initiation.

This PhD project aims to use DNA-based recorders (based on serine integrase) to reconstruct the gene expression programme during lateral root founder cell specification. You will combine this cutting-edge methodology with single-cell RNA sequencing to dissect spatiotemporal gene regulation of lateral root initiation.

You will build and optimise integrase-based genetic circuits in Arabidopsis to record the order in which genes of interest are expressed. Using fluorescence microscopy, you will analyse the resulting Arabidopsis lines to better understand the gene regulatory network underlying lateral root founder cell specification, with high spatiotemporal resolution.

You will also use single-cell RNA sequencing (combined with FACS) to determine the cell state of precise lateral root founder cell subpopulations marked with the engineered genetic circuit. During this project, you will develop your critical thinking and scientific independence, with the final part of the project being up to you to shape.

This project offers:
• Comprehensive training in engineering biology, automation, molecular biology, microscopy, plant transformation, protoplasting, FACS, single-cell RNA sequencing and analysis.
• Opportunities to present your work at international conferences and industry symposia.
• A vibrant, supportive research environment with excellent career progression: past students at the Earlham Institute became biotech CEOs, group leaders, and research scientists.
• A highly collaborative and supportive research community with access to training and career development opportunities across the Norwich Research Park.

You will become an expert in plant engineering biology through this project and take part in the mission of the Guiziou group, which is to have a tangible impact on sustainable agriculture.