Using gene editing and pluripotent stem cells to investigate cardiovascular cell signalling

SMITH_U20DTP

The heart is a multi-cellular organ, with intercellular communication between cardiomyocytes, cardiac fibroblasts, and endothelial cells all necessary to maintain normal cardiac function. It is becoming clear that signalling via extracellular vesicles (EVs) plays important roles in regulation of cardiac function, with dynamic and two-way interactions necessary between the multiple cell populations.

This project will use state-of-the-art gene edited pluripotent stem cell models, to gain new insights relating to the mechanisms involved in regulating cardiac function at rest, and during periods of increased workload. Training will be provided in the areas of stem cell biology, cardiac biology, differentiation, advanced flow cytometry, single cell sorting, RT-qPCR, and CRISPR/Cas9-based genome editing. As well as the specific training detailed above, students will have access to high-quality training in scientific and generic skills, as well as access to a wide-range of seminars and training opportunities.

The project will be carried out at the Smith Lab at the Bob Champion Research and Education building, Norwich Medical School, University of East Anglia. This work will be in close collaboration with the M√ľnsterberg Lab, based at School of Biological Sciences, University of East Anglia.