Talpid3 and endothelial cells

ROBINSON_Q21DTP2

Angiogenesis is the formation of blood vessels from pre-exisiting vessels. Angiogenesis is essential for maintaining healthy physiological processes and for the revascularisation of tissues later in life (e.g. after an injury). In this project you will be investigating the the role of Talpid3, an important component of primary cilia, in regulating angiogenesis in both chick and mouse models.

The goal of this studentship is to determine how endothelial Talpid3 regulates angiogenesis at the molecular, cellular and organismal levels. The project would give the student experience in a wide range of cross-disciplinary techniques, including working with tissue specific knockout mice, micro-injection of gene modulators in chicken embryos, endothelial cell isolation, cell imaging and tissue culture. The student will learn standard techniques such as Western blotting, immunohistochemistry, immunofluorescence and the use of confocal microscopy. The project will also involve culture of endothelial cells and a variety of in vitro signalling and biochemistry assays.

The project offers students the opportunity to work in two different dynamic environments – one at the Quadram Institute and one at the School of Biological Sciences at UEA. Both places offer excellent facilities and resources for undertaking a wide variety of different biomedical research techniques. This opportunity for a studentship is available for exceptional candidates with an interest in cell biology, molecular biology, developmental biology, and/or vascular biology.

References:

Berbari, N. F., O’Connor, A. K., Haycraft, C. J. and Yoder, B. K. (2009). The primary cilium as a complex signaling center. Curr Biol 19, R526-35.

Davey, M. G., Paton, I. R., Yin, Y., Schmidt, M., Bangs, F. K., Morrice, D. R., Smith, T. G., Buxton, P., Stamataki, D., Tanaka, M., M√ľnsterberg, A., Tickle, C. and Burt, D. (2006). The chicken talpid3 gene encodes a novel protein essential for Hedgehog signaling. Genes Dev 20, 1365-77.

Yin, Y., Bangs, F., Paton, I. R., Prescott, A., James, J., Davey, M. G., Whitley, P., Genikhovich, G., Technau, U., Burt, D. W. and Tickle, C. (2009). The Talpid3 gene