Picking apart the complex interactions between integrins and neuropilin-1 during angiogenesis (ROBINSON_Q19DTP)
- Research Area Bioscience for Health
- Partner The Quadram Institute Bioscience (QIB)
- Supervisor Dr Stephen Robinson - email@example.com
- Application Deadline 26/11/2018
Angiogenesis is the formation of blood vessels from pre-existing vessels. Angiogenesis is essential for maintaining healthy physiological processes and for the revascularisation of tissues following an injury. Our research group focuses on investigating the molecular basis of angiogenesis and we have recently identified a novel interaction between three cell surface proteins expressed by endothelial cells involved in this process.
The goal of the studentship would be to determine how endothelial alpha5-integrin, beta3-integrin and neuropilin-1 proteins interact (in an identified complex network) to regulate angiogenesis at the molecular, cellular and organismal levels. This project would give the student experience in a wide range of cross-disciplinary techniques, including working with knockout mice, endothelial cell isolation, cell imaging, and tissue culture. The student would learn standard techniques such as western blotting, immunohistochemistry, immunofluorescence and the use of confocal microscopy. The project would involve culture of endothelial cells and a variety of migration, sprouting and in vitro assays which model angiogenesis. The project aims to use conditional knockout mice to determine the in vivo function of these two proteins during developmental angiogenesis and following a vascular injury, in varied in vivo models
This project offers students an opportunity to work in a dynamic environment, in a research institute with excellent facilities and resources for undertaking a wide variety of biomedical research. This opportunity for a studentship is available for exceptional candidates with an interest in cell biology, molecular biology, developmental biology, and/or vascular biology.