Modulating biological processes using light (SACHDEVA_U19DTP)
- Research Area Frontier Bioscience
- Partner The University of East Anglia (UEA)
Dr Amit Sachdeva -
- Application Deadline 26/11/2018
Site-specific incorporation of non-natural amino acids into proteins could provide new approaches to study and control biological processes. The goal of this project is to develop methods for genetic site-specific incorporation of novel photocaged amino acids into proteins expressed in live cells. Development of these methods will allow gaining spatial and temporal control over several biological processes using light. The research work will involve chemical synthesis of non-natural amino acids, developing directed evolution based approaches to enable genetic encoding of new photocaged amino acids, and application of photocaged amino acids to modulate biological processes.
The successful applicant will be a talented biochemist or chemist with enthusiasm for chemistry, chemical biology, and molecular biology. The candidate will work at the forefront of chemical and synthetic biology and will develop advanced skills in chemical synthesis, various molecular biology and analytical techniques, including molecular cloning, protein expression and purification, gel electrophoresis, western blotting, and HPLC. The research work will be performed in the Chemical and Synthetic Biology laboratories housed in the School of Chemistry, University of East Anglia under the supervision of Dr. Amit Sachdeva.