Gaining photo-control over post-translational modifications (SACHDEVA_U18DTP1)
- Research Area Frontier Bioscience
- Partner The University of East Anglia (UEA)
Dr Amit Sachdeva -
- Application Deadline 27/11/2017
Nature elegantly orchestrates complex chemical and physical transformations to accomplish functions as diverse as transmitting information across generations to harvesting energy from sunlight. Most of these processes are performed by proteins built out of 20 naturally occurring amino acids. However, naturally occurring proteins often require additional chemical functional groups, such as cofactors (metal ions and organic cofactors) and post-translational modifications for their activity. Site-specific incorporation of non-natural amino acids with novel functional groups into proteins synthesised in cells could provide new approaches to study and control biological processes, and also create new biomolecules. The goal of this project is to develop methods for site-specific incorporation of photocaged post-translational modifications into proteins expressed in live cells. This project will allow gaining photocontrol over several biological processes and also has the potential to provide access to medically important proteins that are difficult to access.
The successful applicant will be a talented biochemist or chemist with enthusiasm for chemistry, chemical biology, and molecular biology. The candidate will work at the forefront of chemical and synthetic biology and will develop advanced skills in chemical synthesis, various molecular biology and analytical techniques, including molecular cloning, protein expression and purification, gel electrophoresis, western blotting, and HPLC. The research work will be performed in the Chemical and Synthetic Biology laboratories housed in the School of Chemistry, University of East Anglia under the supervision of Dr. Amit Sachdeva.