From predictions to validation: Functional characterization of splicing in the ADAMTS family during development (HAERTY_E19DTP)
- Research Area Bioscience for Health
- Partner The Earlham Institute (EI)
Dr Wilfried Haerty -
- Application Deadline 26/11/2018
We offer a highly collaborative PhD project between three research groups (Haerty: bioinformatics, Wheeler: cell and developmental biology, Edwards: developmental and molecular biology) combining computational biology and experimental developmental biology to investigate the diversity and functional during Vertebrate development of alternatively spliced transcripts originating from genes of the ADAMTS (A Disintegrin and Metalloproteinase with /thrombospondin type I motifs) family. The PhD student will gain expertise in computational biology, large datasets analysis, transcriptomics, arising long read sequencing technologies, genetics, microscopy and developmental biology gaining highly transferable skills.
Nearly all the genes in human undergo alternative splicing, the process through which different transcripts are generated from a single gene. Despite the observation of the universality of alternative splicing in Eukaryotes, tissue and developmental stage specific regulation of splicing, and the importance of this mechanism in fundamental biological processes, much remain to be discovered regarding the function of alternatively spliced transcripts.
The aim of the project is to provide a novel understanding of the importance and regulation of alternative splicing in the ADAMTS gene family during development. ADAMTS are secreted enzymes with roles in tissue morphogenesis and patho-physiological remodeling. The student will apply combined computational and experimental approaches to annotate and functionally characterize transcripts through in-situ expression assays and gene manipulations in Xenopus.