Can there be only one? Exploring heterogeneity in RNA splicing at single-cell resolution (MACUALAY_E18DTP)
- Research Area Frontier Bioscience
- Partner The Earlham Institute (EI)
Dr Iain Macaulay -
- Application Deadline 27/11/2017
Alternative splicing is a key mechanism by which cells generate functional diversity from individual genes, however, it is not yet will understood if individual cells express a single or multiple alternatively spliced isoforms. In this project, we aim to unravel the complexity of alternative splicing in blood stem cells - to see how these cells regulate expression of individual isoforms as part of their functional heterogeneity,
The successful applicant will develop and apply cutting-edge techniques in single-cell genomics to explore this question. The Macaulay group has world-leading expertise in single-cell genomics and a strong background in blood cell formation and function, and secondary supervisor Wilfried Haerty has expertise in developing bioinformatics tools for the analysis of single-cell data. The student will be trained in both wet- and dry- lab techniques to take a fundamental biological question and develop and apply new tools to provide an unequivocal answer.
The student will gain a unique and influential skill set in cellular genomics, through training in haematopoietic stem cell biology and isolation by (FACS), single-cell genomics, NGS technology development and bioinformatics. The student will work in the exceptionally well equipped labs at EI, within a group that has an excellent track record in novel single-cell technology development and the PI has a strong network of collaborators in haematology (Cambridge, UCL, Karolinska) and genomics (Sanger Institute, KU Leuven) as well as key industrial partners.
The ideal applicant will enjoy solving complex technical problems, be curious about new technologies as well as fundamental questions in biology, and have a willingness to train in new techniques, as well as training others.